PRISM – Protein-RNA Interactions by Structural Methods

The PRISM team (Protein-RNA Interactions by Structural Methods) investigates the functional basis of nucleic-acid binding proteins by collecting data at the atomic level. Our goal is to reveal key steps in fundamental biological processes, and to use these molecular details to determine what goes wrong with these systems during disease. In addition, we explore direct binding of nucleic acids with a variety of biological or synthetic molecules in the context of viral infection and cancer.

Topics :

  • Structural domains in ribosome biogenesis
  • Regulation of tissue-specific alternative splicing
  • Function of folded and intrinsically-disordered proteins in cellular condensates
  • Unusual nucleic acid structures
  • Biomolecular NMR for chemical biology

Approaches :

  • Biomolecular NMR spectroscopy
  • X-ray crystallography
  • Single molecule FRET
  • Recombinant proteins and protein complexes
  • Isothermal titration calorimetry
  • Differential scanning calorimetry